Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen

	Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen
	Xu, Ya-Jie 1; Qiang, Min 1,2; Zhang, Jin-Ling 1; Liu, Ying1 ; He, Rong-Qiao 1,3; He, RQ (reprint author), Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China.
心理所单位排序	1,3
摘要	Fibrinogen is a key protein involved in coagulation and its deposition on blood vessel walls plays an important role in the pathology of atherosclerosis. Although the causes of fibrinogen (fibrin) deposition have been studied in depth, little is known about the relationship between fibrinogen deposition and reactive carbonyl compounds (RCCs), compounds which are produced and released into the blood and react with plasma protein especially under conditions of oxidative stress and inflammation. Here, we investigated the effect of glycolaldehyde on the activity and deposition of fibrinogen compared with the common RCCs acrolein, methylglyoxal, glyoxal and malondialdehyde. At the same concentration (1 mmol/L), glycolaldehyde and acrolein had a stronger suppressive effect on fibrinogen activation than the other three RCCs. Fibrinogen aggregated when it was respectively incubated with glycolaldehyde and the other RCCs, as demonstrated by SDS-PAGE, electron microscopy and intrinsic fluorescence intensity measurements. Staining with Congo Red showed that glycolaldehyde-and acrolein-fibrinogen distinctly formed amyloid-like aggregations. Furthermore, the five RCCs, particularly glycolaldehyde and acrolein, delayed human plasma coagulation. Only glycolaldehyde showed a markedly suppressive effect on fibrinogenesis, none did the other four RCCs when their physiological blood concentrations were employyed, respectively. Taken together, it is glycolaldehyde that suppresses fibrinogenesis and induces protein aggregation most effectively, suggesting a putative pathological process for fibrinogen (fibrin) deposition in the blood.
关键词	fibrinogen acrolein glycolaldehyde glyoxal malondialdehyde methylglyoxal
学科领域	Physiological Psychology/biological Psychology
	2012-08-01
语种	英语
发表期刊	PROTEIN & CELL
ISSN	1674-800X
卷号	3 期号:8 页码:627-640
期刊论文类型	Article
URL	查看原文
收录类别	SCI
项目简介	We thank Dr. Joy Fleming for helpful comments and improving the English of the manuscript. This work was supported by the National Basic Research Program (973 Program) (Grant Nos. 2010CB912303 and 2012CB911004), and the Natural Scientific Foundation of China (Grant Nos. NSFC 30970695 and QBI-IBP GJHZ1131).
WOS记录号	WOS:000310531900009
资助机构	National Basic Research Program (973 Program) [2010CB912303, 2012CB911004] ; Natural Scientific Foundation of China [NSFC 30970695, QBI-IBP GJHZ1131]
引用统计	被引频次：23[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/12909
专题	脑与认知科学国家重点实验室
通讯作者	He, RQ (reprint author), Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China.
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China
第一作者单位	脑与认知科学国家重点实验室
推荐引用方式 GB/T 7714	Xu, Ya-Jie,Qiang, Min,Zhang, Jin-Ling,et al. Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen[J]. PROTEIN & CELL,2012,3(8):627-640.
APA	Xu, Ya-Jie,Qiang, Min,Zhang, Jin-Ling,Liu, Ying,He, Rong-Qiao,&He, RQ .(2012).Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen.PROTEIN & CELL,3(8),627-640.
MLA	Xu, Ya-Jie,et al."Reactive carbonyl compounds (RCCs) cause aggregation and dysfunction of fibrinogen".PROTEIN & CELL 3.8(2012):627-640.

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