Reversal of Cocaine-Conditioned Place Preference through Methyl Supplementation in Mice: Altering Global DNA Methylation in the Prefrontal Cortex

	Reversal of Cocaine-Conditioned Place Preference through Methyl Supplementation in Mice: Altering Global DNA Methylation in the Prefrontal Cortex
	Tian, Weiping 1; Zhao, Mei2 ; Li, Min 5; Song, Tianbao 1; Zhang, Min 3; Quan, Li 3; Li, Shengbin 1; Sun, Zhong Sheng4,5 ; Tian, WP (reprint author), Xi An Jiao Tong Univ, Sch Med, Dept Forens Sci, Xian 710049, Shanxi, Peoples R China.
心理所单位排序	2
摘要	Analysis of global methylation in cells has revealed correlations between overall DNA methylation status and some biological states. Recent studies suggest that epigenetic regulation through DNA methylation could be responsible for neuroadaptations induced by addictive drugs. However, there is no investigation to determine global DNA methylation status following repeated exposure to addictive drugs. Using mice conditioned place preference (CPP) procedure, we measured global DNA methylation level in the nucleus accumbens (NAc) and the prefrontal cortex (PFC) associated with drug rewarding effects. We found that cocaine-, but not morphine- or food-CPP training decreased global DNA methylation in the PFC. Chronic treatment with methionine, a methyl donor, for 25 consecutive days prior to and during CPP training inhibited the establishment of cocaine, but not morphine or food CPP. We also found that both mRNA and protein level of DNMT (DNA methytransferase) 3b in the PFC were downregulated following the establishment of cocaine CPP, and the downregulation could be reversed by repeated administration of methionine. Our study indicates a crucial role of global PFC DNA hypomethylation in the rewarding effects of cocaine. Reversal of global DNA hypomethylation could significantly attenuate the rewarding effects induced by cocaine. Our results suggest that methionine may have become a potential therapeutic target to treat cocaine addiction.
学科领域	Physiological Psychology/biological Psychology
	2012-03-16
语种	英语
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	7 期号:3
期刊论文类型	Article
URL	查看原文
收录类别	SCI
项目简介	This work was supported by Natural Science Foundation of China Grants (30870821 and 81071078), Youth Development Funding (O9KF015001) from the Institute of Psychology and Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
WOS记录号	WOS:000303309100045
资助机构	Natural Science Foundation of China [30870821, 81071078] ; Institute of Psychology and Key Laboratory of Mental Health, Institute of Psychology, Chinese Academy of Sciences
引用统计	被引频次：74[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/13248
专题	中国科学院心理健康重点实验室
通讯作者	Tian, WP (reprint author), Xi An Jiao Tong Univ, Sch Med, Dept Forens Sci, Xian 710049, Shanxi, Peoples R China.
作者单位	1.Xi An Jiao Tong Univ, Sch Med, Dept Forens Sci, Xian 710049, Shanxi, Peoples R China 2.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 3.Capital Inst Pediat, Dept Mol Immunol, Beijing, Peoples R China 4.Chinese Acad Sci, Beijing Inst Life Sci, Beijing, Peoples R China 5.Wenzhou Med Coll, Inst Genom Med, Wenzhou, Peoples R China
推荐引用方式 GB/T 7714	Tian, Weiping,Zhao, Mei,Li, Min,et al. Reversal of Cocaine-Conditioned Place Preference through Methyl Supplementation in Mice: Altering Global DNA Methylation in the Prefrontal Cortex[J]. PLOS ONE,2012,7(3).
APA	Tian, Weiping.,Zhao, Mei.,Li, Min.,Song, Tianbao.,Zhang, Min.,...&Tian, WP .(2012).Reversal of Cocaine-Conditioned Place Preference through Methyl Supplementation in Mice: Altering Global DNA Methylation in the Prefrontal Cortex.PLOS ONE,7(3).
MLA	Tian, Weiping,et al."Reversal of Cocaine-Conditioned Place Preference through Methyl Supplementation in Mice: Altering Global DNA Methylation in the Prefrontal Cortex".PLOS ONE 7.3(2012).

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