Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates

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	Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates
	Pan, Rong 1; Zhang, Xue-Jing 1,2; Zhang, Zi-Jian 1,3; Zhou, Yuan 1,3; Tian, Wei-Xi 3; He, Rong-Qiao 1,3
摘要	Enzymatic catalysis of biochemical reactions is essential to all living systems. The "lock and key" and "induced fit" models were early contributions to our understanding of the mechanisms involved in the reaction between an enzyme and its substrate. However, whether a given substrate-induced conformation is rigid or remains flexible has not yet been determined. By measuring the enzyme activity and intrinsic fluorescence of a nonspecific Eisenia fetida protease-I with different chromogenic substrates, we show that in subsequent reactions of protease with substrates, both the "lock and key" and "induced fit" mechanisms are used depending on the degree of conformational change required. Chromozym-Th-or chromosym-Ch-induced protease conformations were unable to bind chromozym-U. The chromosym-U-induced protease conformation remained flexible and could be further induced by chromozym-Th and chromozym-Ch. When low concentrations of guanidine HCl were used to disturb the conformation of the enzyme, only small changes in intrinsic fluorescence of the chromozym-Th-induced protease were detected, in contrast to the native enzyme whose intrinsic fluorescence markedly increased. This indicates that the substrate-induced enzyme was relatively rigid compared with the native protease. Utilizing a lock and key mechanism for secondary substrate reactions may have adaptive value in that it facilitates high efficiency in enzymatic reactions.
	2010-07-23
语种	英语
发表期刊	JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN	0021-9258
卷号	285 期号:30 页码:22948-22954
期刊论文类型	Article
收录类别	SCI
WOS记录号	WOS:000279999900029
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/13719
专题	中国科学院心理研究所回溯数据库(1956-2010)
作者单位	1.Chinese Acad Sci, Inst Biophys, State Key Lab Brain & Cognit Sci, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
第一作者单位	脑与认知科学国家重点实验室
推荐引用方式 GB/T 7714	Pan, Rong,Zhang, Xue-Jing,Zhang, Zi-Jian,et al. Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2010,285(30):22948-22954.
APA	Pan, Rong,Zhang, Xue-Jing,Zhang, Zi-Jian,Zhou, Yuan,Tian, Wei-Xi,&He, Rong-Qiao.(2010).Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates.JOURNAL OF BIOLOGICAL CHEMISTRY,285(30),22948-22954.
MLA	Pan, Rong,et al."Substrate-induced Changes in Protease Active Site Conformation Impact on Subsequent Reactions with Substrates".JOURNAL OF BIOLOGICAL CHEMISTRY 285.30(2010):22948-22954.