Comparative RNA-seq analysis reveals potential mechanisms mediating the conversion to androgen independence in an LNCaP progression cell model

	Comparative RNA-seq analysis reveals potential mechanisms mediating the conversion to androgen independence in an LNCaP progression cell model
	Wang, Yongqing1,2,3 ; Wang, Yan 1,2,3; Liu, Qi 4; Xu, Gang 5; Mao, Fengbiao 2,6; Qin, Tingting 5; Teng, Huajing 6; Cai, Wanshi 2,4,6; Yu, Ping 4; Cai, Tao 4; Zhao, Mei1,3 ; Sun, Zhong Sheng 1,4,6; Xie, Congying 7
摘要	The androgen-independent phenotype is an important symptom of refractory prostate cancer. However, the molecular mechanisms underlying this phenotypic conversion remain unclear. Using RNA-seq analysis of androgen-dependent prostate cancer cells (LNCaP) vs. androgen-independent cancer cells (LNCaP-AI-F), we identified 788 differentially expressed genes, 315 alternative splicing events, and eight novel LNCaP-AI-F-specific fusion genes. The fusion genes ElF2AK1-ATR and GLYR1-SLC9A8 were predicted to be damaging and oncogenic. We also observed dramatic changes in androgen receptor (AR)-mediated pathway molecules, including prostate-specific antigen (PSA, a major biomarker of prostate cancer) and AR variants, as well as neuroendocrine-like (NE-like) and tumor stem cell-like characteristics, during androgen-independent phenotype progression. Our findings provide new insights into the regulatory complexities of refractory prostate cancers. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
关键词	Prostate cancer Androgen-independent RNA-seq Alternative splicing Fusion gene Tumor stem cell-like
	2014
语种	英语
发表期刊	CANCER LETTERS
ISSN	0304-3835
卷号	342 期号:1 页码:130-138
期刊论文类型	Article
收录类别	SCI
WOS记录号	WOS:000328591100015
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/14105
专题	中国科学院心理健康重点实验室
作者单位	1.Chinese Acad Sci, Inst Psychol, Behav Genet Ctr, Beijing 100101, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Psychol, Key Lab Mental Hlth, Beijing 100101, Peoples R China 4.Wenzhou Med Coll, Inst Genom Med, Wenzhou, Zhejiang, Peoples R China 5.Wenzhou Med Coll, Affiliated Hosp 1, Ctr Clin Lab Diag, Wenzhou, Zhejiang, Peoples R China 6.Chinese Acad Sci, Beijing Inst Life Sci, Beijing 100101, Peoples R China 7.Wenzhou Med Coll, Affiliated Hosp 1, Radiotherapy & Chemotherapy Dept, Wenzhou, Zhejiang, Peoples R China
第一作者单位	健康与遗传心理学研究室; 中国科学院心理健康重点实验室
推荐引用方式 GB/T 7714	Wang, Yongqing,Wang, Yan,Liu, Qi,et al. Comparative RNA-seq analysis reveals potential mechanisms mediating the conversion to androgen independence in an LNCaP progression cell model[J]. CANCER LETTERS,2014,342(1):130-138.
APA	Wang, Yongqing.,Wang, Yan.,Liu, Qi.,Xu, Gang.,Mao, Fengbiao.,...&Xie, Congying.(2014).Comparative RNA-seq analysis reveals potential mechanisms mediating the conversion to androgen independence in an LNCaP progression cell model.CANCER LETTERS,342(1),130-138.
MLA	Wang, Yongqing,et al."Comparative RNA-seq analysis reveals potential mechanisms mediating the conversion to androgen independence in an LNCaP progression cell model".CANCER LETTERS 342.1(2014):130-138.

条目包含的文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
WOS_000328591100015.（1303KB）	期刊论文	出版稿	暂不开放	CC BY-NC-SA	请求全文