Drug dependence has been considered as a reoccurred brain disease characterized by persistent abnormal carving for addictive drug and psychological dependence. Especially, the reconsolidation of the reward and aversion emotional arouse induced by drug is a important reason of the psychology dependence. In the present series of studies, aimed to elucidating the differential neural substrates of the two opposite emotional aroused memory reconsolidation induced by drug, the role of NMDA receptor and β-adrenergic receptors in core of nucleus accumbens(NAC) and basolateral amygdale (BLA) on the reconsolidation of morphine associated reconsolidation were investigated in conditioned place preference (CPP) and conditioned place aversion(CPA) paradigm. The main results are as follows: 1. Reconsolidation of morphine CPA (mCPA) was persistent disrupted after intracerebroventricularly injection of anisomycin, one of protein sythesis inhibitor. Reactiveation with a contextual recall destabilized an established mCPA, but morphine CPP (mCPP) need contextual and drug to reactive. 2.Established mCPP and mCPA are disrupted by injection of inhibition of protein synthesis into the BLA. 3.Microinjection of β-adrenergic receptors antagonist DL-Propranolol into the BLA disrupt the reconsolidation of mCPA, but not mCPP; no effect was abserved when α- adrenergic receptors antagonis Phentolamine and NMDA recptor antagonist D-APV microinjected into BLA. 4.Intra-NAC of anisomycin disrupted reconsolidation of mCPP, but not mCPA. 5.The reconsolidation of mCPP but not mCPA was disrupted by NMDA receptor antagonist D-APV inject into the core of NAC; no disrupt effect was found when microinjection of β-adrenergic receptors antagonist DL-Propranolol into the NAC on reconsolidation of mCPA and mCPP. These results suggest there are different types of memory reactivation between two opposite morphine related emotional memory. Role of β-adrenergic receptors whithin BLA and NMDA receptor within NAC are different on the reconsolidation of mCPA and mCPP. β-adrenergic receptors in BLA mediate the memory reconsoliation of mCPA, NMDA receptor in core of NAC mediate the memory reconsoliation of mCPP.
修改评论