慢性冷水游泳应激对大鼠海马、前脑皮层神经颗粒素含量的影响

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	慢性冷水游泳应激对大鼠海马、前脑皮层神经颗粒素含量的影响
其他题名	The changes of neurogranin level in rat hippocampus and forebra in after chronic cold waterswimming stress
	李欢欢; 林文娟
通讯作者邮箱	[email protected]
摘要	目的:探讨慢性应激对大鼠海马和前脑皮层神经颗粒素(NG)含量的影响。方法:将雄性SD大鼠随机分为应激组(予10℃冷水游泳应激,共2周)、装置对照组和正常对照组,每组10只。于应激前后测量三组大鼠的体质量变化情况,并记录每天应激过程中应激组和装置对照组的排便量以考察应激的强度,应激后以Westernblotting方法测定海马和前脑皮层中的NG含量(以NG含量与βActin含量的比值表示)。结果:(1)应激前后,三组大鼠的体质量比较,差异均无统计学意义(P>0.05),但应激组的体质量增长相对缓慢; (2)应激第4—14天应激组的排便量多于装置对照组(P<0.01); (3)应激组和装置对照组海马的NG含量[分别为(0.66±0.13)和(0.94±0.26)]低于正常对照组[(1.93±0.53)],差异有统计学意义(P<0.01); (4)应激组前脑皮层的NG含量[(0.45±0.00)]低于装置对照组和正常对照组[分别为(2.76±1.74)和(2.87±1.63)],差异有统计学意义(P<0.01); (5)应激组海马NG含量高于前脑皮层(P<0.01); 装置对照组海马NG含量低于前脑皮层(P<0.01); 正常对照组海马与前脑皮层NG含量的差异无统计学意义。结论:慢性应激导致海马和前脑皮层NG含量显著下降; 对于时程长、程度严重、适应不良的冷水游泳应激,前脑皮层受损比海马更为严重; 而对于时程长、程度微弱的装置应激,海马则比前脑皮层更为敏感。
其他摘要	Objective: To investigate the effects of chronic cold water swimming stress on protein levels of neurogranin in rat hippocampus and forebrain, and to understand the central mechanisms of stress from the molecular level.Methods: Thirty SD rats were randomly divided into handled-controls, apparatus-controls and stressed groups, with ten in each group. Forced 10℃ cold water swimming was used as a stressor. The defecation numbers of both stressed rats and apparatus controls were recorded during 5-minute trial on each day. Furthermore, the weight of rats was monitored during the periods of stress as an index reflecting degree of stress. The neurogranin level in the hippocampus and forebrain after stress was measured with the Western blotting. Results:（1） Although there was no significant difference in final body weight among the three groups, less weight gain was in stressed rats than in controls. （2） In comparison with the apparatus controls, the stressed group had significantly more numbers of defecation during a 5-minute trial on the 4th to 14th experiment day （P<0.01）, and no significant differences were found between two groups on the experiment day 1 to 3 （P>0.05）. （3） the hippocampal protein levels of neurogranin in stressed group （0.66±0.13） and apparatus-controls （0.94±0.26） were significantly lower than that in handled-controls （1.93±0.53）（P<0.01）. （4） The forebrain protein levels of neurogranin in stressed group （0.45±0.00） were significantly lower than that in apparatus-controls （2.76±1.74） and handled-controls （2.87±1.63）（P<0.01）. （5） The protein levels of neurogranin in hippocampus were significantly higher than that in forebrain （P<0.01） in stressed group. However, the protein levels of neurogranin in apparatus-controls was significantly higher in forebrain than in hippocampus （P<0.01）. There was no significant difference between forebrain and hippocampal neurogranin levels in handled-controls.Conclusion: Chronic swimming stress can significantly decrease the protein levels of neurogranin in hippocampus and forebrain, with more severe changes in the forebrain than in the hippocampus. However, hippocampus seems to be more sensitive than forebrain in response to chronic mild apparatus stress.
关键词	应激海马前脑大脑皮质神经颗粒素
学科领域	生理心理学
	2005
语种	中文
发表期刊	中华精神科杂志
ISSN	1006-7884
卷号	38 期号:3 页码:174-177
收录类别	CSCD
资助项目	国家自然科学基金资助项目(30370482);中国科学院创新工程资助项目(KSCX222203)
CSCD记录号	CSCD:1995224
引用统计	被引频次：4[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.psych.ac.cn/handle/311026/2788
专题	中国科学院心理研究所回溯数据库(1956-2010)
通讯作者	林文娟
作者单位	中国科学院心理研究所
第一作者单位	中国科学院心理研究所
通讯作者单位	中国科学院心理研究所
推荐引用方式 GB/T 7714	李欢欢,林文娟. 慢性冷水游泳应激对大鼠海马、前脑皮层神经颗粒素含量的影响[J]. 中华精神科杂志,2005,38(3):174-177.
APA	李欢欢,&林文娟.(2005).慢性冷水游泳应激对大鼠海马、前脑皮层神经颗粒素含量的影响.中华精神科杂志,38(3),174-177.
MLA	李欢欢,et al."慢性冷水游泳应激对大鼠海马、前脑皮层神经颗粒素含量的影响".中华精神科杂志 38.3(2005):174-177.

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