The placebo effect is the effect that follows the administration of an inert medical treatment (the placebo), the placebo analgesia response plays an important role in clinical practice. Due to the limit of clinical ethnics trial, the biological mechanisms of placebo responses remain largely unknown. A rat model for placebo analgesia research is firstly established in the present study, specific and non-specific antagonists for opioid receptor are injected into the rostral anterior cingulate cortex (rACC). Pain threshold is measured by hot plate test to determine the main brain regions and types of opioid receptor in placebo analgesia. The endomorphin-1 and 2 in rACC are measured by enzyme-linked immunosorbent assay (ELISA), the aminoglutaminic acid in paraventricular nucleus (PVN) is measured by biochemical method, the mRNA and protein levels of N-methyl D-aspartate receptor (NMDAR) 1, 2A and 2B are measured by real-time RT-PCR and western blot respectively. The type of endogenous opioid, project resources and the relation between endogenous opioid releasing and glu-NMDAR activation are determined by the above experiment. The present research for placebo analgesia is the first time study in animal model. This research will help to discovery the brain mechanism of psychological factors modulating the global expe
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