健康与遗传心理学研究室知识库

【目的】探究抑郁症肝郁脾虚证是否会出现SIRT1/PGC-1α通路的异常。【方法】纳入健康人(平和质)、肝气郁结型抑郁症患者和肝郁脾虚型抑郁症患者各10例,采集外周血以Western Blot法检测3组受试者的沉默信息调节因子1(SIRT1)、过氧化物酶体增殖物激活受体-γ共激活因子1α(PGC-1α)、核呼吸因子1(NRF-1)、核呼吸因子2(NRF-2)、线粒体转录因子A(TFAM)蛋白表达含量。【结果】与健康人组相比,肝气郁结型抑郁症组患者外周血SIRT1蛋白表达量显著升高(P<0.05),肝郁脾虚型抑郁症组患者外周血SIRT1、PGC-1α、TFAM蛋白表达量显著降低(P<0.05)。【结论】SIRT1/PGC-1α通路介导的线粒体能量代谢及生物合成异常可能是抑郁症肝郁脾虚证的生物学实质之一。

To investigate the occurrence of the abnormalities of SIRT1/PGC-1α pathway in depression with liver depression and spleen deficiency syndrome or not. Methods The investigation was carried out in 10 healthy volunteers with balanced constitution， 10 depression patients with liver-qi stagnation syndrome and 10 depression patients with liver depression and spleen deficiency syndrome. The expression levels of silent information regulator 1（SIRT1），peroxisome proliferator-activated receptor γ coactivator 1（PGC-1α），nuclear respiratory factor 1（NRF-1），nuclear respiratory factor 2（NRF-2）， and mitochondrial transcription factor A（TFAM）in the peripheral blood of the 3 groups were detected by Western Blot method. Results Compared with the healthy control group，the expression level of SIRT1 protein in the peripheral blood of depression patients with liver-qi stagnation syndrome was increased significantly（P < 0.05）， while the expression levels of SIRT1， PGC-1α and TFAM protein in the peripheral blood of depression patients with liver depression and spleen deficiency syndrome were decreased significantly（P < 0.05）. Conclusion The abnormal mitochondrial energy metabolism and biosynthesis mediated by SIRT1/PGC-1α pathway may be one of the biological essences of depression with liver depression and spleen deficiency syndrome.