| 其他摘要 | Post-traumatic stress disorder (PTSD) is a mental disorder with complex etiology, which usually occurs after individuals experience or witness traumatic events. Non- coding RNAs (ncRNAs) are RNAs transcribed from the genome, which do not encode proteins. Regulatory ncRNAs can regulate gene expression and participate in physiological and pathological processes, they are closely related to the occurrence and development of human diseases. Studies of ncRNAs of PTSD has increased year by year, emphasizing the important role of ncRNAs in the pathogenesis of PTSD. However, the huge varieties and numbers of ncRNAs and their many-to-many relationship with target genes result in the different association between ncRNAs and PTSD. On the other hand, due to differences of research methods and demographic variables, the heterogeneity among different studies cannot be ignored, and the significant results are difficult to be replicated, which hinders further exploration of the pathological mechanism of PTSD.
This study aims to comprehensively retrieve and integrate studies reported associations between ncRNAs and PTSD, and on this basis, further investigate the role of ncRNAs in regulating PTSD development. 253 articles were screened by reading abstract, 37 ncRNAs researches of PTSD were included in this study eventually. Then, through reading the full articles and manually extracting the study information, such as selection of samples, measurement and diagnosis of PTSD, types of ncRNAs and relevant measuring methods, as well as specific markers and their statistical results, the core data of PTSD-ncRNAs were constructed. The association between some ncRNAs and PTSD has been repeatedly verified, such as miR-19b and miR-193a-5p. Besides, some highly conserved miRNAs showed cross-species consistent association with PTSD, such as let-7d-5p, miR-221, and miR-132. Next, using non-coding RNA databases to retrieve the basic information of the ncRNAs associated with PTSD, and obtain the target gene sets of ncRNAs by species (human, rat, and mouse). Based on ClusterProfiler, package developed by R language, the KEGG and GO enrichment pathway were analyzed for the target gene set of ncRNAs, and results demonstrates that the PTSD ncRNAs are significantly involved in the regulation of cell growth and development, stress response, immune inflammation, and nervous system related pathways. Furthermore, this study explored the overlap biological mechanisms between PTSD and related psychiatric disorders (schizophrenia, major depression, and substance abuse disorder) from the aspect of RNA regulation. Results suggests that the common regulatory mechanisms between PTSD and these related psychiatric disorders are pathways related to hormone regulation and signal transduction. By updating the E- R relation and content of MySQL relational database, the core data of PTSD-ncRNAs information will be presented on the PTSDepigenetics (http://ptsdepigenetics.psych.ac.cn/), providing researchers with quick and convenient retrieval function and related fields of cutting-edge progress.
In this study, the existing ncRNAs studies for PTSD were reviewed and integrated in details, and some insights on the pathogenesis of PTSD were proposed through data mining analysis of ncRNAs associated with PTSD, hoping to provide inspiration and convenience for researchers in related fields. |
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